Karen Crawford, professor of biology, has recently been awarded a Whitman Fellowship from the Marine Biological Laboratory (MBL). The award will be used to cover laboratory space and housing at MBL in Woods Hole, Massachusetts. Crawford will use the award to continue her sabbatical research project investigating the use of CRISPR-Cas9 genome editing to knockout specific gene functions in developing embryos.
The title of her proposal is: Beyond Proof of Concept - using CRISPR-Cas9 genome editing in Doryteuthis pealii embryos to determine: 1) the role of ADAR1 and ADAR2 RNA editing enzymes in development, morphology and behavior; and 2) the impact of Homeobox gene function on embryonic pattern and development.
In addition to the genes mentioned in her proposal title, she will attempt to knock in genes into the cephalopod genome for the first time.